Generator

Part:BBa_I716354:Design

Designed by: Austin Day   Group: iGEM07_Berkeley_UC   (2007-10-24)


Biobricked Unmodified Hemoglobin Cassette in petDUET format


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 87
    Illegal PstI site found at 591
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 591
    Illegal NotI site found at 609
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal BamHI site found at 1394
    Illegal XhoI site found at 1403
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 87
    Illegal PstI site found at 591
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 87
    Illegal PstI site found at 591
    Illegal NgoMIV site found at 1101
    Illegal NgoMIV site found at 1208
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.


Source

Pre-biobricking modifications to I716091. Quickchange AD0013F/R to get rid of EcoR1 site. Quickchange AD0014/F/R to get rid of BglII site. Quickchange AD0015F/R to get rid of XhoI site.

PCR AD0016F/R on modified I716091. Digest Bgl2/Xho1 and insert into 9145 plasmid. Product is I716354

References